Composite

Part:BBa_K777101:Design

Designed by: Team Goettingen   Group: iGEM12_Goettingen   (2012-09-19)

flhDC operon under the control of constitutive promoter J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 650
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer.
  • flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
    • Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3'
      (the capitalized "C" induces the mutation for removal of the PstI site)

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
  • J23100 information was taken from the parts.igem and physical DNA from the 2012 distribution kit.

References